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Passive enhancement and hyperacute rejection of renal allografts in the rat
Attempts to induce passive enhancement of renal allografts with donor-specific cytotoxic antibody can result in antibody-mediated damage to the grafted organ. Hyperacute renal allograft rejection occurred in the homozygous DA to Lewis strain combination when Lewis anti-DA serum was administered with guinea pig complement. Hyperacute rejection was prevented in four of five rats when Lewis anti-DA F(ab')2 was injected before the administration of Lewis anti-DA serum and complement and these grafts had enhanced survival. Red blood cell absorption of the Lewis anti-DA serum to produce an anti-Ia-like serum, also removed its ability to produce hyperacute rejection without removing its ability to enhance. With appropriate modifications both of these techniques would be applicable to human transplantation.
Attempts to induce passive enhancement of renal allografts with donor-specific cytotoxic antibody can result in antibody-mediated damage to the grafted organ. Hyperacute renal allograft rejection occurred in the homozygous DA to Lewis strain combination when Lewis anti-DA serum was administered with guinea pig complement. Hyperacute rejection was prevented in four of five rats when Lewis anti-DA F(ab')2 was injected before the administration of Lewis anti-DA serum and complement and these grafts had enhanced survival. Red blood cell absorption of the Lewis anti-DA serum to produce an anti-Ia-like serum, also removed its ability to produce hyperacute rejection without removing its ability to enhance. With appropriate modifications both of these techniques would be applicable to human transplantation.