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Evaluation of lactobacillus preparation on eterotoxigenic E. coli-induced rabbit ileal loop reactions
The American Journal of Gastroenterology
|March 1, 1980
Summary
This study investigated a lactobacillus preparation for diarrhea treatment. While individual ingredients showed minimal effect, the final product effectively reduced fluid accumulation caused by E. coli enterotoxin.
Area of Science:
- Microbiology
- Gastroenterology
- Pharmacology
Background:
- Lactobacillus preparations are widely used for diarrhea.
- Limited laboratory data exists on their efficacy against enterotoxins.
- The rabbit ileal loop model is suitable for assessing fluid response.
Purpose of the Study:
- To evaluate the efficacy of a commercial lactobacillus preparation against E. coli enterotoxin.
- To determine the contribution of individual ingredients to the preparation's effect.
- To assess the antienterotoxin activity using the rabbit ileal loop model.
Main Methods:
- Enterotoxigenic E. coli were cultured and prepared for injection.
- The lactobacillus preparation and its components were tested in rabbit ileal loops.
- Loop fluid accumulation was measured and compared using the loop fluid ratio (LFR).
Main Results:
- The lactobacillus preparation significantly reduced E. coli enterotoxin-induced fluid accumulation (low LFR).
- Individual ingredients like whey, talc, sugar, evaporated milk, and mineral oil showed variable, often low, antifluid responses.
- The final product demonstrated a significant antienterotoxin effect, unlike its separate components.
Conclusions:
- The commercial lactobacillus preparation possesses significant antienterotoxin activity.
- The efficacy of the preparation is not attributable to its individual ingredients alone.
- Further research into the synergistic effects within the preparation is warranted.