Ly phenotype of lymphocytes producing murine migration inhibitory factor (MIF)

Murine lymphocytes from lymph nodes or peritoneal exudates were tested after treatment with various anti-Ly sera plus complement for their ability to produce MIF in response to stimulation with either antigen or mitogen. When remaining cells in the LN and PEC were stimulated with either Con A or PPD, respectively, it was found that cells of the Lyl set were primarily responsible for MIF production by T cells. This does not rule out the possibility that, under certain circumstances, other T cell sets may produce MIF or material with similar properties after stimulation by antigen.