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Updated: Aug 7, 2026

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
Published on: August 16, 2019
The removal of egg shell membranes by enzyme treatment to facilitate the study of shell microstructure
Abstract:
A procedure is described for the enzymatic hydrolysis of the outer shell membrane of hen eggs using a nonspecific protease. This method is less rigorous than the conventional method of boiling in NaOH and it eliminates the possibility of damage to shell fragments during removal of the adhering membrane.
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