Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Age-related decrease in prostacyclin biosynthetic activity in rat aortic smooth muscle cells

W C Chang, S I Murota, J Nakao

    Biochimica Et Biophysica Acta
    |October 6, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Intake of 25-Hydroxyvitamin D3 Reduces Duration and Severity of Upper Respiratory Tract Infection: A Randomized, Double-Blind, Placebo-Controlled, Parallel Group Comparison Study.

    The journal of nutrition, health & aging·2018
    Same author

    Hip fracture incidence in Japan: Estimates of new patients in 2012 and 25-year trends.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2016
    Same author

    CD30-positive T-cell pseudolymphoma induced by amlodipine.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2008
    Same author

    Development and application of a Japanese model of the WHO fracture risk assessment tool (FRAX).

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2008
    Same author

    Inhibition of T helper 2 chemokine production by narrowband ultraviolet B in cultured keratinocytes.

    The British journal of dermatology·2007
    Same author

    [New diagnostic criteria of primary osteoporosis].

    Clinical calcium·2005

    As rats age, their aortic smooth muscle cells produce less prostacyclin and more prostaglandin E2. This shift in prostaglandin biosynthesis may explain age-related cardiovascular diseases like atherosclerosis and thrombosis.

    Area of Science:

    • Biochemistry
    • Cardiovascular Biology
    • Aging Research

    Background:

    • Prostaglandins play crucial roles in vascular health.
    • Age-related changes in prostaglandin synthesis are implicated in cardiovascular disease pathogenesis.

    Purpose of the Study:

    • To investigate age-dependent prostaglandin biosynthetic activity in rat aortic smooth muscle cells.
    • To determine how aging affects the balance between prostacyclin and prostaglandin E2 production.

    Main Methods:

    • Cultured rat aortic smooth muscle cells from young and old rats were used.
    • Intact cell and cell-free homogenate assays were employed to measure cyclooxygenase activity.
    • Prostaglandin H2 was used as a substrate to assess specific biosynthetic pathways.

    Related Experiment Videos

    Main Results:

    • Total cyclooxygenase activity remained consistent between young and old rat cells.
    • Young rat cells produced more prostacyclin than prostaglandin E2.
    • Old rat cells showed a shift, producing more prostaglandin E2 than prostacyclin.
    • Aged cells exhibited decreased prostacyclin biosynthesis even with prostaglandin H2 as substrate.

    Conclusions:

    • Aging alters the balance of prostaglandin biosynthesis in aortic smooth muscle cells.
    • Reduced prostacyclin and increased prostaglandin E2 in aged rats may contribute to atherosclerosis and thrombosis.
    • These findings offer insights into the molecular mechanisms underlying age-related cardiovascular pathologies.