A longitudinal study of Plasmodium falciparum malaria in the West African savannah using the ELISA technique

Malarial antibody levels were measured by the enzyme-linked immunosorbent assay (ELISA) in two West African populations, one exposed to intense malaria transmission and the other protected. The results reflected the transmission of maternal antibody and, in the unprotected population, the subsequent increase of the ELISA values with age reflected the development of the immune response to malaria. Malaria control activities reduced ELISA values in the protected population. The limitations of the ELISA test used in this study are shown by the fact that numerous infants with previous proven parasitaemia were ELISA-negative. Purified antigens are needed to improve the ELISA test for use in serological surveys of malaria.