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Related Experiment Videos

Quantitation of serum gentamicin concentration by a solid-phase immunofluorescence method

Y Tsay, L Wilson, E Keefe

    Clinical Chemistry
    |October 1, 1980
    PubMed
    Summary

    A novel solid-phase immunofluorescence assay (FIAX) accurately measures gentamicin drug levels in serum or plasma. This method is specific, economical, and avoids radioisotopes, offering a reliable alternative for therapeutic drug monitoring.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Immunology

    Background:

    • Accurate therapeutic drug monitoring is crucial for optimizing antibiotic efficacy and minimizing toxicity.
    • Existing methods for gentamicin quantification, such as radioimmunoassay, involve radioisotopes and complex procedures.

    Purpose of the Study:

    • To develop and validate a solid-phase immunofluorescence assay (FIAX) for the quantitative measurement of gentamicin in biological samples.
    • To evaluate the assay's specificity, sensitivity, and correlation with established methods.

    Main Methods:

    • A solid-phase immunofluorescence method utilizing immobilized anti-gentamicin antibodies on a polymeric surface.
    • Incubation of serum samples with fluorescently labeled gentamicin, followed by fluorescence measurement using a fluorometer.

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  • Comparison of results with radioimmunoassay and radioenzymic assay.

    • Main Results:

    • The FIAX assay demonstrated high specificity for gentamicin, with low cross-reactivity to other drugs (except netilmicin and sisomicin).
    • The assay detected gentamicin concentrations as low as 50 µg/L.
    • Excellent correlation was observed between the FIAX assay and radioimmunoassay (r = 0.94) and radioenzymic assay (r = 0.95).

    Conclusions:

    • The FIAX system provides a specific, sensitive, economical, and user-friendly method for measuring gentamicin concentrations in serum or plasma.
    • This assay offers a safe and efficient alternative to radioisotope-based methods for therapeutic drug monitoring of gentamicin.