Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kidney storage at subzero temperatures using a hyperosmolar perfusate

R Grundmann, B Kaemmerer, D Helling

    European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Obliquity-paced Pliocene West Antarctic ice sheet oscillations.

    Nature·2009
    Same author

    [Urologic errors in surgical procedures].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2008
    Same author

    The authority of complexity.

    The British journal of sociology·2001
    Same author

    Perioperative treatment with filgrastim stimulates granulocyte function and reduces infectious complications after esophagectomy.

    Annals of hematology·2000
    Same author

    [Palliative surgery].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2000
    Same author

    Lessons learnt from multisite pacing for AF management-technical challenges.

    Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·1999
    Same journal

    Two-Stage Fixation in a Rat Model of the Induced Membrane: A Novel Surgical Technique.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    Same journal

    Does treatment of jaundice matter before pancreaticoduodenectomy?: Results from an international multicentre Recurrence After Whipple (RAW) study.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    Same journal

    The Application of Platysma Myocutaneous Flap in Tracheostomy after Total Laryngectomy: A Novel Surgical Technique.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    Same journal

    New Perfusion and Preservation Strategies for Donor Hearts.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    Same journal

    Use of Human Amnion/Chorion Membrane as a Protective Barrier in Complicated Diverticulitis Patients Undergoing Colorectal Resections.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    Same journal

    Erratum.

    European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes·2026
    See all related articles

    Hyperosmolar perfusate effectively preserved dog kidneys for 24 hours. Storage at minus2 degrees C in this solution yielded better results than Collins solution at plus4 degrees C.

    Area of Science:

    • Nephrology
    • Organ Transplantation
    • Preservation Solutions

    Background:

    • Organ preservation is critical for successful transplantation.
    • Current preservation solutions have limitations in extending organ viability.
    • Optimizing perfusate composition and temperature is essential.

    Purpose of the Study:

    • To evaluate the efficacy of a hyperosmolar perfusate for canine kidney preservation.
    • To compare preservation at different temperatures (minus2°C and plus4°C).
    • To assess the impact of human albumin addition to the perfusate.

    Main Methods:

    • 32 canine kidneys were preserved for 12-48 hours.
    • Experimental group: hyperosmolar perfusate (770 mosm/kg) at minus2°C and plus4°C.
    • Control group: Collins solution at plus4°C.

    Related Experiment Videos

  • Post-transplant function assessed via p-aminohippuric acid and inulin clearances.

    • Main Results:

    • Successful 24-hour kidney preservation was achieved with the hyperosmolar perfusate.
    • Kidneys stored at minus2°C in hyperosmolar perfusate showed superior preservation compared to Collins solution at plus4°C.
    • Addition of human albumin did not enhance preservation outcomes.

    Conclusions:

    • Hyperosmolar perfusate is a viable option for extended canine kidney preservation.
    • Lower storage temperatures (minus2°C) combined with hyperosmolar perfusate improve organ viability.
    • Human albumin is not necessary for improving preservation with this hyperosmolar perfusate.