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Phenotypic resistance to miconazole and amphotericin B in Candida albicans

E F Gale, A M Johnson, D Kerridge

    Journal of General Microbiology
    |April 1, 1980
    PubMed

    Insights

    Candida albicans develops resistance to amphotericin B and miconazole due to cell wall changes. These changes differ for each drug, indicating distinct resistance mechanisms.

    Area of Science:

    • Microbiology
    • Mycology
    • Cell Biology

    Background:

    • Candida albicans is an opportunistic fungal pathogen.
    • Antifungal resistance is a growing clinical concern.
    • Cell wall alterations are implicated in fungal drug resistance.

    Purpose of the Study:

    • To investigate the mechanisms of phenotypic resistance to amphotericin B and miconazole in Candida albicans.
    • To determine if cell wall changes are responsible for this resistance.
    • To differentiate the resistance mechanisms for each antifungal drug.

    Main Methods:

    • Culturing Candida albicans under specific growth conditions.
    • Treating fungal cultures with SH-reactive agents.
    • Enzymatic treatment of fungal cell walls.
    • Phenotypic resistance assessment.

    Main Results:

    • Stationary phase cultures of Candida albicans exhibited phenotypic resistance to both amphotericin B and miconazole.
    • This resistance was associated with alterations in the fungal cell wall.
    • Evidence suggests distinct changes in the cell wall are responsible for resistance to amphotericin B versus miconazole.

    Conclusions:

    • Phenotypic resistance in Candida albicans to amphotericin B and miconazole is mediated by cell wall modifications.
    • The specific nature of cell wall alterations differs between amphotericin B and miconazole resistance.
    • Understanding these distinct mechanisms is crucial for developing effective antifungal therapies.

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