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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Published on: July 1, 2011

Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA)

W J Terpstra, G S Ligthart, G J Schoone

    Zentralblatt Fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie
    |August 1, 1980
    PubMed

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    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.