Sperm immunofluorescence in infertile men: Its relationship with sperm agglutination and gel agglutination

The spermatozoa from 10 infertile male patients all with sperm agglutination were subjected to direct immunofluorescence using antisera raised against IgA, IgM, and IgG. Sera from these patients were subjected to the Gel Agglutination Test. Although the Gel Agglutination Test related closely with sperm agglutination, none of the spermatozoa from these patients produced a repeatable positive response to direct immunofluorescence. This study suggests that neither sperm agglutination nor the Gel Agglutination Test demonstrate the presence of sperm specific antibodies in the semen of these infertile men.