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Updated: Aug 17, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Analysis of the fermentation pathways of clostridia using double labelled glutamade
Abstract:
L-(4-14C, 3-3H)Glutamate was used as a tool to elucidate the pathway of its fermentation. The methylaspartate pathway was found in strains of Clostridium tetanomorphum, C. malenominatum, C. limosum, C. lentoputrescens and C. tetani, whereas the hydroxyglutarate pathway was detected in C. sporosphaeroides. These data were supported by measurement of enzyme activities present in cell-free extracts.
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