Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Crystallin expression in the TVI cell line

D S McDevitt, T Yamada

    Differentiation; Research in Biological Diversity
    |April 1, 1980
    PubMed
    Summary

    The newt TVI cell line synthesizes alpha and beta crystallins but lacks gamma crystallins. This suggests iris-derived cells lose lens fiber cell capacity after extensive culturing.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ontogeny and localization of the alpha, beta, and gamma crystallins in newt eye lens development.

    Developmental biology·2010
    Same author

    Fibroblast growth factor receptors and regeneration of the eye lens.

    Developmental dynamics : an official publication of the American Association of Anatomists·1997
    Same author

    Presence and foveal enrichment of rod opsin in the "all cone" retina of the American chameleon.

    The Anatomical record·1993
    Same author

    [A case of staphylococcal enterocolitis caused by methicillin resistant Staphylococcus aureus].

    Hinyokika kiyo. Acta urologica Japonica·1992
    Same author

    [Primary carcinoma in situ of the ureter: a case report].

    Hinyokika kiyo. Acta urologica Japonica·1992
    Same author

    [Significance of normal oral flora, particularly group oral streptococci as defense mechanism against infection in healthy individuals (normal defense mechanism by oral Streptococcus group)].

    Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·1992

    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Ophthalmology

    Background:

    • The TVI cell line originates from newt dorsal iris cells.
    • Iris epithelial cells can differentiate into lens cells in culture.
    • Long-term cell culture may affect differentiated cell functions.

    Purpose of the Study:

    • To investigate crystallin presence in the TVI cell line.
    • To determine if TVI cells retain lens cell synthetic capacity.
    • To assess the impact of passaging on cell differentiation.

    Main Methods:

    • Immunoelectrophoresis to detect crystallins.
    • Immunofluorescence microscopy for cell morphology and crystallin localization.
    • Comparison with known crystallin profiles of amphibian lens cells.

    Main Results:

    • TVI cells contain detectable alpha and beta crystallins.
    • Gamma crystallins were absent in the TVI cell line.
    • A subpopulation of TVI cells resembled lens epithelial cells in crystallin content and morphology.

    Conclusions:

    • The TVI cell line retains some lens cell characteristics, synthesizing alpha and beta crystallins.
    • The absence of gamma crystallins suggests a loss of lens fiber cell differentiation capacity.
    • Prolonged passaging may lead to dedifferentiation or loss of specific synthetic abilities in iris-derived cell lines.

    Related Experiment Videos