A new many-purpose culture medium for chemotherapeutic and microbiologic studies

A new semisynthetic culture medium was prepared and found to serve many useful purposes in chemotherapeutical research and also in diagnostic microbiology. It contains 0.5% peptone, 0.1% glucose and is supplemented with 10% 10% McIlvaine's buffer. The medium is pH-stable and, being almost colorless, it is suitable for turbidimetric studies and enzymatic experiments which involve color-changes. For in vitro chemotherapeutic experimental studies it is a useful medium and, in certain cases, it behaves like an almost antagonist-free medium. Used as broth or in agar-base, it supports the growth of all of the 280 clinically important strains studied, including aerobic bacteria, Candida and Trichophyton. It promotes the germ tube formation of C. albicans and C. stellatoidea.