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Bactericidal screening test for late complement component deficiencies or defects

R H Eng

    Journal of Clinical Microbiology
    |June 1, 1980
    PubMed
    Summary
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    A new, inexpensive assay can detect deficiencies in the late complement components, crucial for fighting infections. This method identified complement deficiencies in four patients, aiding future research into their links with various infections.

    Area of Science:

    • Immunology
    • Microbiology

    Background:

    • Congenital complement deficiencies are linked to Neisseria infections.
    • Their role in other infections is less understood.
    • Traditional CH50 testing is time-consuming and complex.

    Purpose of the Study:

    • To develop an easy, inexpensive method for detecting late complement component deficiencies.
    • To screen patients for these deficiencies.

    Main Methods:

    • An agar pour plate assay using serum-sensitive Escherichia coli.
    • Wells in the agar are filled with patient serum.
    • Absence of bacterial growth inhibition indicates a deficiency.

    Main Results:

    • The assay successfully identified four patients with late complement deficiencies.

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  • One patient had C5 deficiency, and three had C6 deficiency.
  • The method is easy, inexpensive, and effective.

    • Conclusions:

    • This assay is a valuable screening tool for late complement component deficiencies.
    • It can facilitate studies on the link between complement deficiencies and infections.
    • Further research is warranted, especially for partially serum-sensitive organisms.