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Cell surface DNA in immunosuppression in AKR leukemia
Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1980
Summary
Leukemic mouse spleen cells suppress normal immune responses, with DNA-positive cells being key. These DNA-positive cells interact with other cell types to enhance immune suppression.
Area of Science:
- Immunology
- Cell Biology
- Cancer Research
Background:
- Leukemic AKR mice possess spleen cells exhibiting nonspecific T suppressor activity.
- These suppressor cells inhibit the in vitro anti-sheep red blood cell (anti-SRBC) responses of normal AKR spleen cells.
Purpose of the Study:
- To characterize the nature and function of T suppressor cells in leukemic AKR mice.
- To investigate the role of surface DNA and I-J antigens in T suppressor cell activity.
Main Methods:
- Enrichment of suppressor cells using DNase treatment and anti-DNA antibodies.
- Separation of spleen cell populations based on surface DNA and I-J antigen expression.
- Assessment of suppressor cell activity through in vitro anti-SRBC response assays.
Main Results:
- DNase treatment abrogated suppression, indicating the involvement of DNA.
- DNA-positive (DNA+) cells were enriched for suppressor activity, but DNA-negative (DNA-) cells also retained suppressive capacity.
- Sequential fractionation revealed DNA+/I-J- cells as the most potent suppressors, with DNA+/I-J+ and DNA-/I-J+ cells showing lesser activity.
- DNA+/I-J- cells demonstrated synergistic suppression when combined with DNA+/I-J+ and DNA-/I-J+ cells.
Conclusions:
- T suppressor cells in leukemic AKR mice are characterized by surface DNA.
- A subpopulation of DNA+/I-J- cells represents the primary suppressor population.
- Co-culture experiments suggest complex interactions between different cell subpopulations leading to enhanced immune suppression.