An immunoanalysis for kojic acid

An enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of kojic acid in aqueous solutions and biological fluids. As little as 100 pg kojic acid ml-1 was detected in standard buffer. Analysis of biological fluids containing 1 mg kojic acid ml-1 showed that ELISA could detect the toxin at dilutions of 10(-4) in urine, deporteinated plasma, or serum. Urine and plasma samples collected form rabbits dosed intravenously with 56 mg kojic acid kg-1 body weight gave positive tests at dilutions of 10(-4). The immunoassay described may be used for detection of kojic acid produced during experimental or clinical aspergillosis.