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Monocyte cytotoxicity was found to be very dependent on selection of rabbit complement. Eluates obtained from kidney allografts were found to contain a variety of antibodies against monocytes, B cells, and T cells. Specific monocyte reactions were not blocked by an antiserum against human DR antigens made in chickens. A turkey serum against beta 2-microglobulin did abolish monocyte-specific reactions. Blocking with chicken anti-DR was used to assess antibodies in 56 alloantisera. Among the reactions observed, 52% were blocked by the anti-DR serum; 25% appeared to be monocyte-specific.
Monocyte cytotoxicity was found to be very dependent on selection of rabbit complement. Eluates obtained from kidney allografts were found to contain a variety of antibodies against monocytes, B cells, and T cells. Specific monocyte reactions were not blocked by an antiserum against human DR antigens made in chickens. A turkey serum against beta 2-microglobulin did abolish monocyte-specific reactions. Blocking with chicken anti-DR was used to assess antibodies in 56 alloantisera. Among the reactions observed, 52% were blocked by the anti-DR serum; 25% appeared to be monocyte-specific.