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A new selective agent for eukaryotic cloning vectors

J Davies, A Jimenez

    The American Journal of Tropical Medicine and Hygiene
    |September 1, 1980
    PubMed
    Summary
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    The antibiotic G418 inhibits eukaryotes, but a bacterial resistance gene can be introduced into yeast to confer resistance. This combination offers a new tool for recombinant DNA studies and eukaryotic cloning selection.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Aminoglycoside antibiotic G418 inhibits eukaryotic cell growth.
    • A bacterial R-plasmid determinant inactivates G418 through phosphorylation.

    Purpose of the Study:

    • To investigate the utility of a bacterial G418 resistance gene in eukaryotic systems.
    • To explore the potential of this system as a selection marker in recombinant DNA technology.

    Main Methods:

    • Introduction of a bacterial R-plasmid determinant into yeast via transformation.
    • Assessment of yeast resistance to G418 antibiotic.

    Main Results:

    • Successful transformation of yeast with the bacterial resistance gene.

    Related Experiment Videos

  • Expression of G418 resistance in the modified yeast strains.

    • Conclusions:

    • A bacterial G418 resistance mechanism can be functionally expressed in yeast.
    • This antibiotic-resistance system presents a viable dominant selection marker for eukaryotic recombinant DNA studies.