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Hemolytic complement activity in normal human donor corneas

B J Mondino, D B Hoffman

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |November 1, 1980
    PubMed
    Summary
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    This study quantifies hemolytic complement activity in human corneas, finding that molecular weight influences complement component concentration. These findings establish normal values for corneal complement activity, aiding research into corneal diseases.

    Area of Science:

    • Ophthalmology
    • Immunology
    • Biochemistry

    Background:

    • The complement system plays a crucial role in immune responses, including inflammation and tissue damage.
    • Understanding complement component levels in ocular tissues is vital for diagnosing and treating corneal diseases.

    Purpose of the Study:

    • To determine the hemolytic complement activity of specific complement components (C1, C4, C2, C3, C5, C6, C7) in normal human corneas.
    • To compare corneal complement activity with that found in normal human sera.
    • To establish baseline values for complement activity in corneas for future research.

    Main Methods:

    • Normal human corneas were minced and eluted to isolate complement components.
    • Hemolytic complement activity was measured using the 50% hemolysis (CH50) assay with sensitized sheep red blood cells (RBCs).

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  • Corneal eluates were compared to sera from ten normal volunteers.

    • Main Results:

    • The study quantified hemolytic complement activity for C1 through C7 in corneal eluates.
    • Mean hemolytic activity in corneas was compared to mean activity in sera for each complement component.
    • Results suggest that molecular weight may influence the concentration of complement components within the cornea.

    Conclusions:

    • This research provides the first normal values for hemolytic complement activity in human corneas.
    • The established normal values are essential for investigating complement consumption in various corneal diseases.
    • Findings indicate a potential correlation between complement component molecular weight and corneal concentration.