Related Experiment Video
Updated: Aug 9, 2026
Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis
Published on: August 2, 2013
A simple staining method for blood vessels in flat preparations of ocular tissues
Abstract:
We describe a technique for light microscopic visualization of the vasculature of the retina, choroid, ciliary body, and iris in flat preparations. The technique is based on the intravenous injection of horseradish peroxidase, the histochemical demonstration of this enzyme's activity, and the bleaching of the melanin that hinders the visualization of the injected vessels. This technique is reproducible and much simpler than other available methods in which dyes suspended in different media are injected directly into the ocular vessels.
Related Concept Videos
01:03Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
01:24Simple Staining Technique