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Updated: Aug 14, 2026

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Published on: March 14, 2016
Partition affinity ligand assay (PALA). A new approach to binding assays
Abstract:
A two-phase partitioning technique was used to separate free from bound labelled ligand in a binding assay. Efficient separation was accomplished by modifying one of the reactants in such a way that it partitions preferentially to one of the two phases. The biospecific interactions between the lectin concanavalin A and various carbohydrates and glycoproteins were investigated in this model study.
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