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A radioisotopic leukocyte adherence test
Journal of Clinical & Laboratory Immunology
|September 1, 1980
Summary
This study presents a modified leukocyte adherence inhibition test using labeled cells in microwells. The reproducible method shows good correlation with skin testing results for immune responses.
Area of Science:
- Immunology
- Cellular immunology
Background:
- The leukocyte adherence inhibition (LAI) assay is a method to assess cellular hypersensitivity.
- Existing LAI methods can be subjective and difficult to reproduce.
Purpose of the Study:
- To describe a modified, objective, and reproducible leukocyte adherence inhibition assay.
- To evaluate the utility of this modified assay for detecting immune responses to antigens.
Main Methods:
- A modified LAI test was developed using 51Cr-labeled blood mononuclear cells in microwells.
- The assay utilizes approximately 2,000 cells per well, allowing for multiple antigen replicates.
- Inactivated AB serum was found to be optimal for the cell culture medium.
Main Results:
- The modified LAI test demonstrated reproducibility and objectivity.
- Both increases and decreases in leukocyte adherence were observed with tested antigens (SK-SD and PPD).
- A strong correlation was found between the results of this modified LAI test and positive skin tests.
Conclusions:
- The described modified LAI assay is a reproducible and objective method for assessing cellular immune responses.
- This assay shows promise as a reliable alternative to traditional skin testing for immune evaluation.