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Structure and evolution of ribosomes

H G Wittmann

    Molecular Biology, Biochemistry, and Biophysics
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    This study details the structure and assembly of E. coli ribosomes, revealing insights into protein biosynthesis. It highlights differences between prokaryotic and eukaryotic ribosomal proteins despite similar functions.

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    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Structural Biology

    Background:

    • Ribosomes are essential for protein biosynthesis in all organisms.
    • The E. coli ribosome comprises three RNAs and 53 proteins, with 49 primary sequences determined.

    Purpose of the Study:

    • To investigate the structure, assembly, and function of E. coli ribosomes.
    • To compare prokaryotic and eukaryotic ribosomal protein homology.

    Main Methods:

    • Immune electron microscopy and cross-linking to determine ribosomal architecture.
    • Biochemical and genetic analysis of E. coli mutants.
    • Comparative electrophoretic, immunological, and protein-chemical studies.

    Main Results:

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  • Detailed knowledge of ribosomal subunit shape and protein surface localization.
  • Elucidation of cross-linked amino acids and nucleotides in situ.
  • Successful reconstitution of active E. coli 50S ribosomal subunits in vitro.
  • Identification of significant differences in ribosomal proteins between prokaryotes and eukaryotes.

    • Conclusions:

    • In vitro ribosome reconstitution mirrors in vivo assembly.
    • Ribosomal protein alterations in mutants provide insights into ribosome structure and function.
    • Little homology exists between prokaryotic and eukaryotic ribosomal proteins, a surprising finding given their conserved function.