Related Experiment Video

Updated: Aug 16, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Solid-phase enzyme immunoassay of IgM-class rheumatoid factor: comparison of three methods for preparation of the

B Ziola, H Tuokko

    Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
    |June 1, 1980
    PubMed

    Three solid-phase forms of human IgG were compared for their ability to function as the binding target in an enzyme immunoassay for IgM-class rheumatoid factor (RF). IgG was either directly adsorbed to polystyrene beads (method A), or immunologically (method B) or covalently (method C) bound to protein adsorbed to beads. The data presented indicate that C is the method of choice for the preparation of the RF assay solid-phase IgG.

    More Related Videos

    Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay06:15

    Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

    Published on: September 7, 2018

    Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)07:10

    Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

    Published on: April 21, 2019

    Related Concept Videos

    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.