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Effect of antiobiotics on elimination of mycoplasma from experimentally infected cells
Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
|May 1, 1980
Abstract:
The primary and continuous cell cultures and mouse peritoneal macrophages were experimentally infected by Mycoplasma pneumoniae and M. orale I strains and maintained in culture media containing high concentrations of tetracycline or kanamycin. The viable mycoplasmas were eliminated from cell supernatants but not from cells.
Insights
High antibiotic concentrations eliminated viable mycoplasmas from cell culture supernatants but not from within the cells. This study investigated Mycoplasma pneumoniae and M. orale I infections in cell cultures and macrophages.
Area of Science:
- Microbiology
- Cell Biology
- Infectious Diseases
Background:
- Mycoplasma pneumoniae and Mycoplasma orale I are significant respiratory pathogens.
- Understanding their interaction with host cells is crucial for developing effective treatments.
- Antibiotic resistance in Mycoplasma species poses a therapeutic challenge.
Purpose of the Study:
- To investigate the efficacy of high-concentration tetracycline and kanamycin in eliminating Mycoplasma pneumoniae and Mycoplasma orale I from infected cell cultures and macrophages.
- To determine if antibiotics can eradicate intracellular Mycoplasma infections.
Main Methods:
- Primary and continuous cell cultures and mouse peritoneal macrophages were infected with Mycoplasma pneumoniae and Mycoplasma orale I.
- Infected cultures were maintained in media supplemented with high concentrations of tetracycline or kanamycin.
- Cell supernatants and cells were analyzed for the presence of viable mycoplasmas.
Main Results:
- Viable mycoplasmas were eradicated from the cell supernatants of treated cultures.
- However, mycoplasmas were not eliminated from within the infected cells.
- This suggests an intracellular survival mechanism for Mycoplasma species despite antibiotic treatment.
Conclusions:
- High-dose tetracycline and kanamycin are effective in clearing extracellular Mycoplasma from cell cultures.
- Intracellular mycoplasmas remain protected from antibiotic action.
- Further research is needed to develop strategies targeting intracellular Mycoplasma infections.