Related Experiment Video
Updated: Feb 21, 2026

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
Published on: August 8, 2025
A survey on the formation and localization of secondary isozymes in mammalia
Abstract:
A survey on the formation of secondary isozymes (= multiple molecular forms of enzymes) is given by means of well-documented enzyme systems. Further examples of a certain type of formation are summarized in tabular form. Eight different classes of enzyme variants deriving from translational processes are discussed. These are: aggregation, polymerization, oxidation and reduction of free SH groups, limited proteolysis, cleavage of carbohydrate residues, deamidation, noncovalent binding of coenzymes, and conformational isomerism. In addition, the intracellular distribution of secondary isozymes is discussed, as are the formation of artificial enzyme variants and the recognition of multiple enzyme forms caused by an exchange of neutral amino acids. About 200 original papers are cited. The reference list was completed in early 1979.
More Related Videos
07:39Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
Published on: April 13, 2022
07:54Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
Related Concept Videos
Protein Import into the Peroxisomes
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
General Transcription Factors
Ribozymes
Ribozymes can...
Peroxisomes
Overview of Myosin Structure and Function