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Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion
From bovine mammary secretion during the dry period, the total number of cells was between 1.2 and 5.9 X 10(6)/ml. A mean of 35% of these cells were classified as lymphocytes and approximately 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was approximately 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.
From bovine mammary secretion during the dry period, the total number of cells was between 1.2 and 5.9 X 10(6)/ml. A mean of 35% of these cells were classified as lymphocytes and approximately 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was approximately 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.