[Detection and assay of antitetanus antibodies by an immunoenzymatic method]

We report here a sandwich immunoenzymatic assay to detect and determine the amount of antibodies against tetanus toxoid. The procedure used for coating the polystyrene microplates allows the measurement of antibodies in undiluted serum, in one hour. 1100 sera and 80 plasmas were tested. These results show the reproductibility of this simple and automated method. An objective quantification and a rapid screening of sera containing 4 IU/ml or more of antitoxin by direct observation or by measuring the optical density are possible.