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Spontaneous antibody-secreting human blood lymphocytes detected with a protein A plaque assay
Spontaneous antibody-secreting lymphocytes in human peripheral blood were detected and counted by a protein A plaque assay. The level of plaque-forming cells (PFC) in peripheral blood was significantly higher in patients with immune disorders (per 10(6) lymphocytes: IgG PFC, 2,654 +/- 684; IgA PFC, 1,727 +/- 327; IgM PFC, 403 +/- 92) than in normal controls (per 10(6) lymphocytes: IgG PFC, 327 +/- 56; IgA PFC 633 +/- 128; IgM PFC, 215 +/- 105). There was no direct correlation between the PFC numbers of different Ig classes.
Spontaneous antibody-secreting lymphocytes in human peripheral blood were detected and counted by a protein A plaque assay. The level of plaque-forming cells (PFC) in peripheral blood was significantly higher in patients with immune disorders (per 10(6) lymphocytes: IgG PFC, 2,654 +/- 684; IgA PFC, 1,727 +/- 327; IgM PFC, 403 +/- 92) than in normal controls (per 10(6) lymphocytes: IgG PFC, 327 +/- 56; IgA PFC 633 +/- 128; IgM PFC, 215 +/- 105). There was no direct correlation between the PFC numbers of different Ig classes.