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Related Experiment Videos

Visualization of nucleosomes in thin sections by stereo electron microscopy

A L Olins, D E Olins, H Zentgraf

    The Journal of Cell Biology
    |December 1, 1980
    PubMed
    Summary
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    Researchers visualized nucleosomes in chicken red blood cells using electron microscopy. This study demonstrates the stability of nucleosome structure in dispersed chromatin during sample preparation for microscopy.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biophysics

    Background:

    • Nucleosomes are the fundamental structural units of chromatin.
    • Understanding nucleosome structure is crucial for comprehending DNA packaging and gene regulation.
    • Previous visualization methods have faced challenges in preserving native chromatin structure.

    Purpose of the Study:

    • To visualize individual nucleosomes in isolated chicken erythrocytes.
    • To assess the stability of nucleosome structure under specific preparation conditions.
    • To provide high-resolution images of chromatin in a dispersed state.

    Main Methods:

    • Isolation of chicken erythrocytes.
    • Cell lysis and fixation in low ionic strength buffers.
    • Thin sectioning (0.1 micrometer) for electron microscopy.

    Related Experiment Videos

  • High-magnification stereo electron microscopy.

    • Main Results:

    • Nucleosomes were clearly visualized in thin sections of erythrocytes.
    • Low ionic strength buffers maintained chromatin as dispersed filaments.
    • Supranucleosomal structure reformation was prevented during preparation.
    • Nucleosome structure stability was confirmed during fixation, dehydration, and embedding.

    Conclusions:

    • The employed method allows for clear visualization of nucleosomes.
    • The study confirms the stability of nucleosome structure in dispersed chromatin.
    • This technique provides a reliable approach for studying chromatin ultrastructure.