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Related Experiment Videos

Cell surface molecules of Friend erythroleukemias: decrease in T200 glycoprotein expression after induction

R Hyman, I Trowbridge, K Cunningham

    Journal of Cellular Physiology
    |December 1, 1980
    PubMed
    Summary
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    Friend erythroleukemia cells synthesize less T200 glycoprotein when induced, but this reduction is not essential for hemoglobin synthesis or terminal differentiation. T200 glycoprotein expression is not required for Friend erythroleukemia differentiation.

    Area of Science:

    • Hematology
    • Cell Biology
    • Immunology

    Background:

    • Friend erythroleukemias are mouse hematopoietic cell lines used to study differentiation.
    • Cell surface glycoproteins like Thy-1 and T200 play roles in cell function and differentiation.
    • Understanding the regulation of these molecules is crucial for deciphering differentiation pathways.

    Purpose of the Study:

    • To investigate the expression and synthesis of Thy-1 and T200 glycoproteins in Friend erythroleukemia cells.
    • To determine the role of T200 glycoprotein in the induction of hemoglobin synthesis and terminal differentiation.
    • To analyze the relationship between T200 glycoprotein synthesis and cell differentiation.

    Main Methods:

    • Utilized monoclonal antibodies against Thy-1 and T200 glycoproteins.

    Related Experiment Videos

  • Studied cell surface molecule expression on mouse hematopoietic cell lines.
  • Analyzed T200 glycoprotein synthesis rates in induced and noninduced Friend erythroleukemia cells.
  • Isolated and analyzed T200 glycoprotein-negative cell lines using cytotoxic immunoselection.

    • Main Results:

    • Friend erythroleukemia cells express T200 but not significant Thy-1.
    • Maximal induction of Friend erythroleukemia cells reduced T200 glycoprotein synthesis to less than 10% of noninduced levels.
    • T200 glycoprotein expression is reduced in dimethylsulfoxide-selected variant cell lines.
    • T200 glycoprotein-negative cell lines could still undergo hemoglobin synthesis and terminal differentiation.

    Conclusions:

    • Reduction in T200 glycoprotein synthesis during induction is dissociable from commitment and hemoglobin synthesis.
    • The presence of T200 glycoprotein on the cell surface is not necessary for Friend erythroleukemia terminal differentiation.
    • T200 glycoprotein expression is not a prerequisite for Friend erythroleukemia differentiation into hemoglobin-producing cells.