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Related Experiment Videos

Rapid screening for bacteriuria by light scatter photometry (Autobac): a collaborative study

D C Hale, D N Wright, J E McKie

    Journal of Clinical Microbiology
    |January 1, 1981
    PubMed
    Summary

    The Autobac system accurately detects bacteriuria in urine specimens, with high sensitivity for significant bacterial counts within 3-6 hours. It effectively identifies negative specimens, though some false positives occur with lower counts or antimicrobial use.

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    Web watch.

    Hospital medicine (London, England : 1998)·2000

    Area of Science:

    • Clinical Microbiology
    • Diagnostic Technology

    Background:

    • Bacteriuria detection is crucial for diagnosing urinary tract infections.
    • Rapid and accurate diagnostic methods are needed to improve patient management.

    Purpose of the Study:

    • To evaluate the performance of the Autobac system for detecting bacteriuria in urine specimens.
    • To compare Autobac results with standard colony counts.

    Main Methods:

    • A total of 2,720 urine specimens were analyzed using the Autobac system.
    • Results were compared with simultaneous urine colony counts.
    • Specimen analysis included evaluation of detection times (3h and 6h).

    Main Results:

    • Autobac detected 93.8% of specimens with >= 10(5) CFU/ml within 6 hours, increasing to 97% for non-antimicrobial-treated patients.

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  • 77.9% of positive specimens were detected within 3 hours.
  • 98.1% of specimens with < 10(5) CFU/ml were correctly identified as negative by Autobac at 3 hours, decreasing to 86.0% at 6 hours.

    • Conclusions:

    • The Autobac system demonstrates high accuracy for detecting significant bacteriuria.
    • The system shows good performance in identifying negative specimens, with fewer false positives at 3 hours.
    • Factors like antimicrobial treatment and lower bacterial counts can influence Autobac results.