High affinity binding of horseradish peroxidase to collagenous tissue in formalin-paraffin processed human tissue

Horseradish peroxidase or peroxidase conjugated immunoglobulin binds strongly to the collagen in formalin fixed, paraffin embedded human tissue sections. The binding reaction is neither immunological nor organ specific, and may cause confusion in the interpretation of immunoperoxidase reactions in tissue sections containing collagen. This binding reaction can also be useful in identifying collagen fibers in paraffin processed tissue sections.