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Leucocyte mobilization to skin lesions
Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
|October 1, 1980
Summary
This study shows that a skin chamber technique quantifies leucocyte (white blood cell) mobilization to inflammatory sites. Autologous serum concentration and zymosan-treated serum significantly impact leucocyte migration, primarily neutrophils.
Area of Science:
- Immunology
- Dermatology
- Cellular Biology
Background:
- Understanding leucocyte (white blood cell) mobilization is crucial for studying inflammatory responses.
- Existing methods for quantifying leucocyte migration in vivo can be complex.
Purpose of the Study:
- To investigate leucocyte mobilization to a skin lesion using a novel chamber technique.
- To determine factors influencing leucocyte migration and characterize the kinetics of this process.
Main Methods:
- Healthy volunteers were recruited for the study.
- 111Indium-labeled blood leucocytes were tracked in a skin chamber model.
- Variations in autologous serum concentration and zymosan-treated serum were tested.
Main Results:
- Leucocyte accumulation in the chamber confirmed blood-to-lesion mobilization.
- Autologous serum concentration and zymosan-activated serum significantly affected leucocyte counts.
- A consistent mobilization pattern was observed, with peak migration at 20-24 hours, predominantly neutrophil granulocytes.
Conclusions:
- The skin chamber technique offers a simple and quantifiable method to assess in vivo leucocyte mobilization.
- This method allows for the study of leucocyte migration to inflammatory skin lesions.