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Related Experiment Videos

Enkephalinase activity in rat peripheral organs

C Llorens, J C Schwartz

    European Journal of Pharmacology
    |January 5, 1981
    PubMed
    Summary

    Enzyme activity that breaks down [3H]Leu5-enkephalin was found in peripheral organs. This enkephalinase activity is notably high in exocrine and endocrine glands, exceeding brain levels.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Neuroscience

    Background:

    • Enkephalins are endogenous opioid peptides with diverse physiological roles.
    • Enkephalin-degrading enzymes play a crucial role in regulating enkephalin signaling.
    • Particulate fractions from various tissues are used to study enzyme activity.

    Purpose of the Study:

    • To investigate the presence and distribution of enkephalin-hydrolyzing activity in peripheral organs.
    • To characterize the enzyme responsible for the hydrolysis of ([3H]Leu5)-enkephalin.
    • To compare enkephalinase activity in peripheral tissues with that in the brain.

    Main Methods:

    • Incubation of particulate fractions from different peripheral organs with radiolabeled ([3H]Leu5)-enkephalin.
    • Detection and quantification of the resulting tripeptide ([3H]Tyr-Gly-Gly) hydrolysis product.
    • Enzyme activity assays using tissue homogenates.

    Main Results:

    • Hydrolysis of ([3H]Leu5)-enkephalin to [3H]Tyr-Gly-Gly was detected in particulate fractions from numerous peripheral organs.
    • Specific tissues, including exocrine and endocrine glands, exhibited significant enkephalinase activity.
    • Enkephalinase activity in certain peripheral organs surpassed that observed in brain tissue.

    Conclusions:

    • Peripheral organs possess enzymatic machinery for enkephalin degradation.
    • Enkephalinase activity is not confined to the central nervous system and is abundant in specific peripheral tissues.
    • These findings suggest potential peripheral roles for enkephalin metabolism and signaling.

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