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Related Experiment Videos

An improved embedding method for electron microscopy of lepromata

S Okada, Y Fukunishi, A Mukherjee

    International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
    |December 1, 1980
    PubMed
    Summary

    Spurr's resin is the best embedding material for electron microscopy of leprosy. Using dimethylformamide or dimethylsulfoxide enhances the preservation of leprosy bacilli ultrafine structures.

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    Area of Science:

    • Microscopy
    • Pathology
    • Materials Science

    Background:

    • Leprosy research requires high-resolution imaging of pathological tissues.
    • Traditional embedding materials like methacrylate and Epon 812 have limitations in preserving ultrafine structures.

    Purpose of the Study:

    • To identify the optimal embedding medium for electron microscopic examination of lepromata.
    • To improve the preservation of leprosy bacilli ultrastructure during electron microscopy.

    Main Methods:

    • Comparative analysis of Spurr's resin, methacrylate, and Epon 812 for embedding lepromata.
    • Evaluation of different substituting agents (propylene oxide, dimethylformamide, dimethylsulfoxide) for Spurr's resin.

    Main Results:

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  • Spurr's resin demonstrates superior embedding properties for lepromata, comparable to methacrylate for penetration and Epon 812 for stability.
  • Dimethylformamide or dimethylsulfoxide as substitutes in Spurr's resin significantly enhance the preservation of leprosy bacilli ultrafine structures.

    • Conclusions:

    • Spurr's resin mixture is the current optimal embedding material for electron microscopic studies of lepromata.
    • The use of dimethylformamide or dimethylsulfoxide as substitutes further refines Spurr's resin for superior ultrastructural preservation of leprosy bacilli.