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[Antigenic structure of the plasma membranes of mouse liver cells. III. Lipid haptens]
The total glycolipids, gangliosides, neutral lipids and some individual lipids of these fractions were isolated from the whole lipid extract of the mouse liver cell suspension. The antigenic activity of the isolated lipids was studied with the complement fixation test using the rabbit antisera against the liver cell ghosts. Active lipids were: glycosphingolipids, gangliosides, cardiolipin and fraction containing polar glyco- and phospholipid mixture. The cardiolipins isolated from animal cells of some species differed in their antigenic activity. With the immunofluorescence method, the absorbtion of antighost sera by lipid haptens resulted in a decreased fluorescence of plasma membranes of cells in the liver sections. This fact may evidence for the membrane localization of the studied haptens.
The total glycolipids, gangliosides, neutral lipids and some individual lipids of these fractions were isolated from the whole lipid extract of the mouse liver cell suspension. The antigenic activity of the isolated lipids was studied with the complement fixation test using the rabbit antisera against the liver cell ghosts. Active lipids were: glycosphingolipids, gangliosides, cardiolipin and fraction containing polar glyco- and phospholipid mixture. The cardiolipins isolated from animal cells of some species differed in their antigenic activity. With the immunofluorescence method, the absorbtion of antighost sera by lipid haptens resulted in a decreased fluorescence of plasma membranes of cells in the liver sections. This fact may evidence for the membrane localization of the studied haptens.