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Antigen density in ELISA; effect on avidity dependency
Two different densities of an antigen (bovine serum albumin, BSA) were used on paper disks is an enzyme-linked immunosorbent assay (ELISA) for chicken IgG anti-BSA antibodies. Disks of different antigen density but containing the same amount of the antigen showed a difference in immunoreactivity. Samples containing large amounts of low avidity antibodies gave higher absorbance when disks of lower antigen density were used. This phenomenon was not due to a competition between antibodies of various immunoglobulin classes. These findings may indicate that antigen density affects the sensitivity of ELISA to antibodies of various avidities.
Two different densities of an antigen (bovine serum albumin, BSA) were used on paper disks is an enzyme-linked immunosorbent assay (ELISA) for chicken IgG anti-BSA antibodies. Disks of different antigen density but containing the same amount of the antigen showed a difference in immunoreactivity. Samples containing large amounts of low avidity antibodies gave higher absorbance when disks of lower antigen density were used. This phenomenon was not due to a competition between antibodies of various immunoglobulin classes. These findings may indicate that antigen density affects the sensitivity of ELISA to antibodies of various avidities.