Prostaglandins and renin release studies in the isolated perfused rat kidney

The efficacy of various prostaglandins to stimulate renin release was compared in the isolated perfused rat kidney. Renin release was stimulated in decreasing order of potency by PGE2, PGE2, PGF2 alpha and PGI2, whereas PGA2, PGD2 and 6-keto-PGF1 alpha were without effect. Renovascular resistance (RVR) was increased following infusion of PGE2, PGE1 and PGF2 alpha, whereas PGI2 did not change RVR. When PGF2 alpha or 3H-PGF2 alpha was infused, PGE2 could be identified in the venous effluent by high-performance liquid chromatography and combined gas chromatography/mass spectrometry.