Peptidase activities in the functioning jejunum and ileum before and after jejunoileal bypass in morbid obesity

Different peptidase activities were studied preoperatively and postoperatively on intestinal biopsy specimens from patients with jejunoileal bypass for morbid obesity. Preoperatively the activities of the brush border peptidases, microvillus aminopeptidase (EC 3.4.11.2) and dipeptidyl peptidase IV (EC 3.4.14.X), were lowest at the ligament of Treitz and highest in the distal ileum. The activity of the other brush border peptidase studied, the gamma-glutamyl transpeptidase (EC 2.3.2.2), had a maximum at proximal jejunum, like the two cytosol dipeptidases glycyl-leucine dipeptidase (EC 3.4.13.2) and proline dipeptidase (EC 3.4.13.9). Postoperative changes in peptidase activities were most pronounced in the ileal part of the shunt, whereas changes were small in the jejunal part. The most conspicuous finding was a significant increase in gamma-glutamyl transpeptidase activity in the ileum. There was also tendency to an increase of the cytosol dipeptidases in the ileum. In contrast, the activity of microvillus aminopeptidase and dipeptidyl peptidase IV in the ileum had a tendency to decrease.