Binding of biosynthetic human insulin to human antibodies and receptors

The binding of biosynthetic human insulin (BHI) and pork insulin to anti-pork insulin antibodies was tested at an insulin concentration of 4 microunits/ml. Identical binding data were obtained. The binding of the two insulins to mononuclear lymphocytes was also identical. The data are compared to previous results obtained with synthetic human insulin. Previously, we investigated the in vitro properties of fully synthetic human insulin from Dr. Rittel, Ciba-Geigy, Basel. Receptor binding of this human insulin was identical to pork insulin. The binding properties of the new BHI from Lilly (Indianapolis) were studied with human antibodies and freshly isolated human monocytes.