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Bartonella bacilliformis: colonial types and erythrocyte adherence

T S Walker, H H Winkler

    Infection and Immunity
    |January 1, 1981
    PubMed
    Summary

    Bartonella bacilliformis adheres to human red blood cells, with one type showing double the adherence. This bacterial adherence to erythrocytes is likely mediated by glycolipids and requires bacterial energy.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Infectious Diseases

    Background:

    • Bartonella bacilliformis is a bacterium known to infect humans.
    • Understanding the interaction between Bartonella bacilliformis and human erythrocytes is crucial for comprehending its pathogenesis.

    Purpose of the Study:

    • To differentiate and characterize colony types of Bartonella bacilliformis.
    • To investigate the adherence mechanisms of Bartonella bacilliformis to human erythrocytes.
    • To identify the erythrocyte components involved in Bartonella bacilliformis adherence.

    Main Methods:

    • Cultivation of Bartonella bacilliformis and differentiation into colony types T1 and T2.
    • In vitro adherence assays using human erythrocytes.
    • Treatment of erythrocytes with enzymes (alpha- or beta-glucosidase, pronase, subtilisin) to probe adherence mechanisms.

    Main Results:

    • Two distinct colony types (T1 and T2) of Bartonella bacilliformis were identified.
    • Bartonella bacilliformis adhered to human erythrocytes in vitro, with T2 exhibiting approximately twice the adherence of T1.
    • Adherence was energy-dependent, suggesting a role for motility, and was mediated by erythrocyte glycolipids, as indicated by enzyme treatment studies.

    Conclusions:

    • Bartonella bacilliformis exhibits differential adherence to human erythrocytes based on colony morphology.
    • Bacterial energy and motility are essential for maximal adherence.
    • The adherence of Bartonella bacilliformis to erythrocytes is likely mediated by glycolipid structures on the erythrocyte surface.

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