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Related Experiment Videos

A simple, rapid and sensitive assay for immune complexes using a Staphylococcus aureus immunoadsorbent

T Barkas

    Journal of Clinical & Laboratory Immunology
    |January 1, 1981
    PubMed
    Summary
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    A new assay detects immune complexes using Staphylococcus aureus and radiolabeled protein A, simplifying detection in autoimmune disorders and cancer. This method is sensitive and rapid, avoiding cell lines or protein purification.

    Area of Science:

    • Immunology
    • Biochemistry
    • Assay Development

    Background:

    • Immune complexes (ICs) are implicated in various autoimmune diseases and cancers.
    • Accurate detection of ICs is crucial for diagnosis and monitoring.
    • Existing assays often require complex procedures, cell lines, or protein purification.

    Purpose of the Study:

    • To develop a simple, rapid, and sensitive assay for detecting immune complexes.
    • To establish a method that does not necessitate cell line maintenance or protein purification.
    • To validate the assay's performance and applicability in clinical samples.

    Main Methods:

    • Preferential precipitation of complexed immunoglobulin.
    • Binding of precipitated complexes to Staphylococcus aureus.

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  • Detection using radiolabeled protein A.

    • Main Results:

    • The assay readily detects 3-6 µg/ml of aggregated IgG in normal human serum.
    • Detects soluble in vitro produced complexes over a wide range (4 to >100 fold excess of antigen).
    • Identified immune complexes in sera from patients with autoimmune disorders and lung cancer, with two distinct higher molecular weight peaks in SLE serum.

    Conclusions:

    • The described assay is simple, rapid, and sensitive for detecting immune complexes.
    • The method is effective in identifying ICs in patient sera, including those with autoimmune diseases and cancer.
    • The assay is robust, with interference from free immunoglobulin or anti-S. aureus antibodies ruled out.