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Growth of Mycobacterium lepraemurium in cell-free liquid medium containing soluble starch: evaluation by generation
Abstract:
A stimulatory effect of soluble starch on the growth of M. lepraemurium in vitro cell-free culture system has been noted. In this medium bacteria elongated gradually without manifesting any significant peak in elongation as has been noted in NC-5 medium. The maximum average elongation of bacteria was 2.84 mu on the 24th day in NCS-5 medium. In contrast, in NC-5 bacteria elongated maximum on the 6th day (2.32 mu). All the stages of bacterial cell division were noted. Hypothesis has been suggested for continuous multiplication of M. lepraemurium in NCS-5 medium.