Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cybrid formation with recipient cell lines containing dominant phenotypes

R W Yatscoff, J R Mason, H V Patel

    Somatic Cell Genetics
    |January 1, 1981
    PubMed
    Summary

    Researchers developed a new method for creating cybrids using drug-resistant Chinese hamster ovary (CHO) cells. This technique enhances cybrid clone frequency by utilizing dominant drug-resistance markers for efficient cell line selection.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Choice between food and cocaine or fentanyl reinforcers under fixed and variable schedules in female and male rhesus monkeys.

    Psychopharmacology·2023
    Same author

    The acute, systemic effects of aerobic exercise in recently concussed adolescent student-athletes: preliminary findings.

    European journal of applied physiology·2022
    Same author

    Getting in Front of Chronic Wasting Disease: Model-Informed Proactive Approach for Managing an Emerging Wildlife Disease.

    Frontiers in veterinary science·2021
    Same author

    Quantification of observable behaviors induced by typical and atypical kappa-opioid receptor agonists in male rhesus monkeys.

    Psychopharmacology·2020
    Same author

    Self-administration of benzodiazepine and cocaine combinations by male and female rhesus monkeys in a choice procedure: role of α1 subunit-containing GABA<sub>A</sub> receptors.

    Psychopharmacology·2019
    Same author

    The α2,3-selective potentiator of GABA<sub>A</sub> receptors, KRM-II-81, reduces nociceptive-associated behaviors induced by formalin and spinal nerve ligation in rats.

    Pharmacology, biochemistry, and behavior·2019

    Area of Science:

    • Cell Biology
    • Genetics
    • Biochemistry

    Background:

    • Mitochondrial inheritance and drug resistance are crucial in cell biology.
    • Developing efficient methods for cybrid formation is essential for studying cytoplasmic inheritance and mitochondrial function.
    • Previous methods for cybrid selection often relied on recessive markers, limiting efficiency.

    Purpose of the Study:

    • To isolate a novel drug-resistant Chinese hamster ovary (CHO) cell line for use in cybrid formation.
    • To develop an improved method for cybrid selection using dominant drug-resistance markers.
    • To increase the efficiency of cybrid clone generation.

    Main Methods:

    • Isolation of a Tevenel-resistant CHO cell clone (BT3) with mitochondrial-level, cytoplasmic inheritance.

    Related Experiment Videos

  • Development of a cybrid formation protocol using a DRB-resistant cell line as the nuclear recipient.
  • Removal of nucleated cells from cytoplasts via unipore filters prior to fusion.
  • Selection of cybrids based on dominant DRB and Tevenel resistance phenotypes.

    • Main Results:

    • Successfully isolated BT3, a CHO cell clone resistant to Tevenel at the mitochondrial level.
    • Established a novel cybrid formation method utilizing dominant nuclear (DRB) and cytoplasmic (Tevenel) resistance markers.
    • Achieved a 16-fold increase in cybrid clone frequency compared to methods using recessive markers.
    • Demonstrated the efficiency of immediate cybrid selection post-fusion.

    Conclusions:

    • The developed method significantly enhances cybrid selection efficiency.
    • The use of dominant drug-resistance markers offers a powerful tool for cybrid formation.
    • This approach holds potential for application in recipient cells lacking selectable markers.