'Non-specific' binding of lac repressor of E. coli to a carcinogen-modified DNA

The 'non-specific' binding of lac repressor to a N-hydroxyaminofluorene (N-OH-AF) modified DNA was studied using circular dichroism. The binding process was not altered by the modification of the DNA. A small increase of the binding constant was observed. These results are discussed with respect to the role of carcinogen modification in the protein-nucleic acid recognition phenomena.