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Related Experiment Videos

Digoxin sensitivity in amyloid cardiomyopathy

A Rubinow, M Skinner, A S Cohen

    Circulation
    |June 1, 1981
    PubMed
    Summary
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    Amyloid fibrils bind digoxin, a medication used for heart conditions. This interaction may explain why some patients with amyloid cardiomyopathy are sensitive to digitalis drugs.

    Area of Science:

    • Cardiology
    • Biochemistry
    • Pharmacology

    Background:

    • Amyloid cardiomyopathy is a serious condition.
    • Patients with this condition sometimes show increased sensitivity to digitalis medications like digoxin.
    • The underlying mechanism for this sensitivity is not fully understood.

    Purpose of the Study:

    • To investigate the interaction between amyloid fibrils and digoxin.
    • To determine if amyloid fibrils can bind digoxin.
    • To explore the potential role of this binding in digitalis sensitivity in amyloid cardiomyopathy.

    Main Methods:

    • Incubation of purified amyloid fibrils and tissue homogenates with digoxin.
    • Separation of bound and unbound digoxin via centrifugation and washing.

    Related Experiment Videos

  • Quantification of digoxin concentration in supernatants.
  • Detection of bound digoxin using immunofluorescence assays with antidigoxin antiserum.

    • Main Results:

    • Isolated amyloid fibrils demonstrated significant binding of digoxin (0.11–0.13 ng/ml per milligram of fibrils).
    • Bound digoxin could not be removed by repeated washing or changes in buffer pH.
    • Immunofluorescence confirmed the presence of digoxin bound to amyloid fibrils and in amyloid heart homogenates.

    Conclusions:

    • Amyloid fibrils possess the ability to bind digoxin.
    • This binding interaction suggests a potential mechanism for digoxin accumulation or altered pharmacokinetics in amyloid cardiomyopathy.
    • The binding may contribute to the observed digitalis sensitivity in some patients with this condition.