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Related Experiment Videos

Rapid screening for bacteriuria using a particle counter, pulse-height analyser, and computer

M K Alexander, M S Khan, C S Dow

    Journal of Clinical Pathology
    |February 1, 1981
    PubMed
    Summary

    This study presents a rapid urine screening system for bacteriuria using a Coulter Counter and ultrasound. The system achieves high sensitivity for detecting bacterial infections, improving diagnostic speed.

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    Area of Science:

    • Clinical Microbiology
    • Medical Diagnostics
    • Biophysics

    Background:

    • Bacteriuria screening is crucial for diagnosing urinary tract infections.
    • Rapid and sensitive diagnostic methods are needed to improve patient outcomes.
    • Current methods can be time-consuming, delaying treatment.

    Purpose of the Study:

    • To develop and evaluate a rapid screening system for bacteriuria.
    • To assess the efficacy of ultrasound treatment in enhancing detection sensitivity.
    • To determine the system's performance in identifying bacterial growth and abnormal cyturia.

    Main Methods:

    • Utilized a Coulter Counter Model ZBI with a multichannel pulse-height analyzer (Coulter "Channelyser").
    • Employed computer analysis for processing pulse-height data.

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  • Applied brief low-intensity ultrasound treatment (5-10 seconds) to undiluted urine specimens.

    • Main Results:

    • Achieved 99.1% sensitivity for single-pathogen bacteriuria (>100 x 10^6/l).
    • Detected 85-90% of mixed bacterial growths (>10 x 10^6/l), with sonication showing no improvement.
    • Ultrasound significantly increased detection of abnormal cyturia without bacterial growth (33% to 72%).

    Conclusions:

    • The Coulter Counter-based system offers a rapid and sensitive method for bacteriuria screening.
    • Ultrasound sonication enhances the detection of abnormal cyturia, potentially indicating non-bacterial causes.
    • This approach can expedite the diagnosis of urinary tract conditions.