Immunological properties of bulk isolated mouse oligodendrocytes

Mouse oligodendrocytes were isolated in bulk. These cells were 95% galactocerebroside-positive. Rabbit anti-mouse oligodendrocyte antisera reacted in radioimmunoassay and in indirect immunofluorescence with mouse oligodendrocytes in suspension or in culture. The activity of the sera was not blocked by adsorption with galactocerebroside or by preincubation of cells with anti-galactocerebroside serum, although it cross-reacted with galactocerebroside in a serological test. The staining was not impaired by the pretreatment of cells with trypsin, neuraminidase or acetone, nor by adsorption of serum with myelin basis protein.